| Final 19:24 | time | gap | L | React. | 50m | | 1. Nicholas Thoman |  USA | 53.20s | PR | 5 | 0.64 | 27.52s | 1. | | 2. Guilherme Guido |  BRA | 53.35s | 0.15 | 4 | 0.59 | 27.57s | 2. | | 3. Eugene Godsoe | USA | 53.96s | 0.76 | 3 | 0.60 | 27.56s | 5. | | 4. Russell Wood |  CAN | 54.30s | 1.10 | 6 | 0.55 | 27.91s | 4. | | 5. Federico Grabich |  ARG | 54.61s | 1.41 | 2 | 0.62 | 28.30s | 3. | | 6. Omar Pinzon |  COL | 55.22s | 2.02 | 7 | 0.58 | 28.66s | 6. | | 7. Markus Thormeyer | CAN | 55.52s | 2.32 | 8 | 0.71 | 28.16s | 8. | | 8. Albert Subirats |  VEN | 56.09s | 2.89 | 1 | 0.60 | 29.42s | 7. |
| World Record: Aaron Piersol (USA) 51.94s, Indianapolis (USA), 08.07.2009. | | Pan American Games Record: Randal Bal (USA) 53.66s, Rio de Janeiro (BRA), 22.07.2007. | | Qualification times. Standard A: 56.59s, Standard B: 59:99. |
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| Final B 19:19 | time | gap | L | React. | 50m | | 9. Armando Barrera |  CUB | 56.14s | | 4 | 0.81 | 28.85s | 2. | | 10.Daniel Torres |  MEX | 56.91s | 0.77 | 2 | 0.59 | 29.55s | 3. | | 11.Charles Hockin |  PAR | 57.25s | 1.11 | 5 | 0.59 | 29.75s | 4. | | 12.Luis Martinez |  GUA | 57.30s | 1.16 | 6 | 0.60 | 29.50s | 6. | | 13.Timothy Wynter |  JAM | 57.47s | 1.33 | 7 | 0.63 | 29.86s | 5. | | 14.Robinson Molina | VEN | 57.92s | 1.78 | 3 | 0.59 | 30.73s | 1. | | 15.Christopher Courtis |  BAR | 57.93s | 1.79 | 1 | 0.63 | 30.12s | 7. | | 16.Matthew Mays |  ISV | 59.14s | 3.00 | 8 | 0.61 | 30.55s | 8. |
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Heats - Friday 17.07 10:15 | | First 8 to Final. Next 8 to Final B. |
| Ranking | time | gap | L | React. | 50m | Heat | | 1. Guilherme Guido | BRA | 54.04s | Q | 4 | 0.60 | 27.76s | 1. | Heat 2 | | 2. Nicholas Thoman | USA | 54.35s | 0.31 | 4 | 0.80 | 27.63s | 1. | Heat 3 | | 3. Eugene Godsoe | USA | 54.38s | 0.34 | 5 | 0.57 | 27.70s | 3. | Heat 2 | | 4. Russell Wood | CAN | 54.55s | 0.51 | 4 | 0.62 | 27.65s | 3. | Heat 1 | | 5. Federico Grabich | ARG | 54.87s | 0.83 | 5 | 0.62 | 28.35s | 2. | Heat 1 | | 6. Omar Pinzon | COL | 54.94s | 0.90 | 3 | 0.60 | 28.38s | 2. | Heat 2 | | 7. Albert Subirats | VEN | 55.24s | 1.20 | 3 | 0.61 | 28.74s | 1. | Heat 1 | | 8. Markus Thormeyer | CAN | 55.26s | 1.22 | 3 | 0.72 | 28.38s | 2. | Heat 3 | | to Final B | | 9. Armando Barrera | CUB | 56.14s | 2.10 | 6 | 0.55 | 29.15s | 3. | Heat 3 | | 10.Charles Hockin | PAR | 56.56s | 2.52 | 2 | 0.61 | 29.43s | 4. | Heat 3 | | 11.Robinson Molina | VEN | 57.08s | 3.04 | 6 | 0.56 | 29.71s | 4. | Heat 1 | | 12.Luis Martinez | GUA | 57.22s | 3.18 | 2 | 0.60 | 29.43s | 5. | Heat 1 | | 13.Daniel Torres | MEX | 57.38s | 3.34 | 6 | 0.55 | 29.88s | 4. | Heat 2 | | 14.Timothy Wynter | JAM | 57.86s | 3.82 | 7 | 0.63 | 29.89s | 5. | Heat 3 | | 15.Christopher Courtis | BAR | 58.15s | 4.11 | 2 | 0.65 | 29.72s | 5. | Heat 2 | | 16.Matthew Mays | ISV | 59.15s | 5.11 | 7 | 0.58 | 30.62s | 6. | Heat 1 | | eliminated | | -. Jordan Augier |  LCA | DNS | | 7 | | | | Heat 2 | | -. Thiago Pereira | BRA | DNS | | 5 | | | | Heat 3 |
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| Swimmer | Q. time | Birth | | Nicholas Thoman | USA | 53.46s | 06 MAR 1986 | | Guilherme Guido | BRA | 53.73s | 12 FEB 1987 | | Russell Wood | CAN | 53.96s | 04 MAY 1994 | | Thiago Pereira | BRA | 54.38s | 26 JAN 1986 | | Eugene Godsoe | USA | 54.42s | 20 JAN 1988 | | Federico Grabich | ARG | 55.15s | 26 MAR 1990 | | Markus Thormeyer | CAN | 55.37s | 25 AUG 1997 | | Omar Pinzon | COL | 55.50s | 17 JUN 1989 | | Albert Subirats | VEN | 55.64s | 25 SEP 1986 | | Armando Barrera | CUB | 56.38s | 18 NOV 1995 | | Daniel Torres | MEX | 56.47s | 09 JUN 1991 | | Robinson Molina | VEN | 56.50s | 05 MAY 1997 | | Standard A: 56.59s | | Charles Hockin | PAR | 56.89s | 04 NOV 1989 | | Christopher Courtis | BAR | 58.37s | 13 FEB 1994 | | Luis Martinez | GUA | 58.57s | 11 DEC 1995 | | Timothy Wynter | JAM | 58.97s | 16 JAN 1996 | | Jordan Augier | LCA | 59.46s | 14 NOV 1994 | | Standard B: 59.99s | | Matthew Mays | ISV | 1:00.38 | 15 DEC 1998 |
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