| Final | time | | | 1. Alvin Kraenzlein |  United States | 25.4s | OR | | 2. Norman Pritchard |  India | 26.0s | | | 3. Walter Tewksbury | United States | 26.1s | | | 4. Eugène Choisel |  France | Unknown | |
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| | | Venue: Bois de Boulogne. 11 competitors from 5 nations. |
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| | | First 2 from each of 2 Heats advanced to Final. |
| Heat 1 | time | | | 1. Alvin Kraenzlein | United States | 27.0s | Q, OR | | 2. Eugène Choisel | France | 27.5s | Q | | 3. Frederick Moloney | United States | 27.6s | | | 4. William Remington | United States | Unknown | | | 5. Gustav Rau |  Germany | Unknown | |
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| Heat 2 | time | | | 1. Norman Pritchard | India | 26.8s | Q, Or | | 2. Walter Tewksbury | United States | 27.1s | Q | | 3. Thaddeus McClain | United States | 27.2s | | | 4. Henri Tauzin | France | Unknown | | | 5. William Lewis | United States | Unknown | | | 6. Zoltán Speidl |  Hungary | Unknown | |
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