| Final | time | | | 1. Maxie Long |  United States | 49.4s | OR | | 2. William Holland | United States | 49.6s | | | 3. Ernst Schultz |  Denmark | 51.5s | | | -. Dixon Boardman | United States | DNS | | | -. Harry Lee | United States | DNS | | | -. William Moloney | United States | DNS | |
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| | | Venue: Bois de Boulogne. 15 competitors from 6 nations. | | Heats |
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Heats - Saturday, 14th July 1900 | | First 2 from each of 3 Heats advanced to Final. |
| Heat | time | | | 1. Maxie Long | United States | 50.4s | Q,OR | | 2. Harry Lee | United States | Unknown | Q | | 3. Harvey Lord | United States | Unknown | | | 4. Georges Clément |  France | Unknown | | | 5. Walter Drumheller | United States | Unknown | |
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| Heat 2 | time | | | 1. William Moloney | United States | 51.0s | Q | | 2. Ernst Schultz | Denmark | Unknown | Q | | 3. Charles-Robert Faidide | France | Unknown | | | 4. Koppán Pál |  Hungary | Unknown | | | 5. Yngvar Bryn |  Norway | Unknown | |
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| Heat | time | | | 1. Dixon Boardman | United States | 51.2s | Q | | 2. William Holland | United States | Unknown | Q | | 3. Henry Slack | United States | Unknown | | | 4-5. Zoltán Speidl | Hungary | Unknown | | | 4-5. Umberto Colombo |  Italy | Unknown | |
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