| Final - played at Crystal Palace, London 15:30 | | date | Winner | score | Finalist | HT | att. | | Sat 20.04.01 | Tottenham Hotspur (SL) | 2-2 | Sheffield United | 1-1 | 114,815 | | 0-1 Alfred Priest 10' 1-1 Alexander Brown 23' 2-1 Alexander Brown 51' 2-2 Walter Bennet 52' Referee: A. Kingscott. | | Replay - Burnden Park, Bolton | | Sat 27.04.01 | Tottenham Hotspur (SL) | 3-1 | Sheffield United | 0-0 | 20,470 | | 1-0 John Cameron 52' 2-0 Tom Smith 76' 2-1 Alfred Priest 40' 3-1 Alexander Brown 51' Referee: A. Kingscott. |
| date | team 1 | score | team 2 | HT | | Mon 08.04.01 | Tottenham Hotspur (SL) | 4-0 | West Bromwich Albion | | | played at Villa Park, Birmingham. | | Sat 06.04.01 | Sheffield United | 2-2 | Aston Villa | | | played at City Ground, Nottingham. | | Replay | | Thu 11.04.01 | Sheffield United | 3-0 | Aston Villa | | | played at Baseball Ground, Derby. |
| date | team 1 | score | team 2 | HT | DN | | Sat 23.03.01 | Reading (qSL) | 1-1 | Tottenham Hotspur (SL) | | 1 | | Sat 23.03.01 | Middlesbrough (II) | 0-1 | West Bromwich Albion | | 3 | | Sat 23.03.01 | Small Heath (II) | 0-0 | Aston Villa | 0-0 | 4 | | Sat 23.03.01 | Wolverhampton Wanderers | 0-4 | Sheffield United | | 2 | | Replays | | Wed 27.03.01 | Aston Villa | 1-0 | Small Heath (II) | | 4r | | Thu 28.03.01 | Tottenham Hotspur (SL) | 3-0 | Reading (qSL) | | 1r |
| date | team 1 | score | team 2 | HT | DN | | Sat 23.02.01 | Tottenham Hotspur (SL) | 2-1 | Bury | | 8 | | Sat 23.02.01 | Bolton Wanderers | 0-1 | Reading (qSL) | | 3 | | Sat 23.02.01 | Woolwich Arsenal (II) | 0-1 | West Bromwich Albion | 0-1 | 7 | | Sat 23.02.01 | Middlesbrough (qII) | 5-0 | Kettering (SL) | | 4 | | Sat 23.02.01 | Small Heath (II) | 1-0 | Burnley | | 5 | | Sat 23.02.01 | Aston Villa | 0-0 | Nottingham Forest | 0-0 | 2 | | Sat 23.02.01 | Notts County | 2-3 | Wolverhampton Wanderers | | 1 | | Sat 23.02.01 | Sheffield United | 2-0 | Everton | | 6 | | Replay | | Wed 27.02.01 | Nottingham Forest | 1-3 | Aston Villa | | 2r |
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| | | Byes to this Round - 16 from Football League Division I, 3 from Football League Division II and 3 from Southern League. |
| date | team 1 | score | team 2 | HT | DN | | Sat 09.02.01 | Tottenham Hotspur (SL) | 1-1 | Preston North End | | 16 | | Sat 09.02.01 | The Wednesday | 0-1 | Bury | | 8 | | Sat 09.02.01 | Reading (qSL) | 2-0 | Bristol Rovers (qSL) | | 4 | | Sat 09.02.01 | Bolton Wanderers | 1-0 | Derby County | | 9 | | Sat 09.02.01 | West Bromwich Albion | 1-0 | Manchester City | | 12 | | Sat 09.02.01 | Woolwich Arsenal (II) | 2-0 | Blackburn Rovers | | 15 | | Sat 09.02.01 | Kettering (qSL) | 1-1 | Chesterfield (II) | | 1 | | Sat 09.02.01 | Middlesbrough (qII) | 3-1 | Newcastle United | | 11 | | Sat 09.02.01 | Newton Heath (II) | 0-0 | Burnley (II) | | 14 | | Sat 09.02.01 | Stoke | 1-1 | Small Heath (II) | | 3 | | Sat 09.02.01 | Nottingham Forest | 5-1 | Leicester Fosse (II) | | 6 | | Sat 09.02.01 | Aston Villa | 5-0 | Millwall Athletic (SL) | | 7 | | Sat 09.02.01 | Notts County | 2-0 | Liverpool | | 5 | | Sat 09.02.01 | Wolverhampton Wanderers | 5-1 | New Brighton Tower (II) | | 10 | | Sat 09.02.01 | Southampton (SL) | 1-3 | Everton | | 2 | | Sat 09.02.01 | Sunderland | 1-2 | Sheffield United | | 13 | | Replays | | Wed 13.02.01 | Chesterfield (qII) | 1-2 | Kettering (qSL) | | 1r | | Wed 13.02.01 | Small Heath (II) | 2-1 | Stoke | | 3r | | Wee 13.02.01 | Burnley (II) | 7-1 | Newton Heath (II) | | 14r | | Wed 13.02.01 | Preston North End | 2-4 | Tottenham Hotspur (SL) | | 16r |
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| | | Liverpool and Stoke from Football League Division I started from this Round. |
| date | team 1 | score | team 2 | HT | DN | | Sat 05.01.01 | Chesterfield (qII) | 3-0 | Walsall (qII) | | 1 | | Sat 05.01.01 | Darwen (qLanc) | 0-2 | Woolwich Arsenal (II) | | 2 | | Sat 05.01.01 | Kettering (qSL) | 1-0 | Crewe Alexandra (q) | | 3 | | Sat 05.01.01 | Stoke | 1-0 | Glossop (II) | | 4 | | Sat 05.01.01 | Reading (qSL) | 1-1 | Bristol City (SL) | | 5 | | Wed 09.01.01 | Bristol City (SL) | 0-0 | Reading (qSL) | | 5r | | Tue 14.01.01 | Reading (qSL) | 2-1 | Bristol City (SL) | | 5r | | Sat 05.01.01 | Grimsby Town (II) | 0-1 | Middlesbrough (qII) | | 6 | | Sat 05.01.01 | Burslem Port Vale (II) | 1-3 | New Brighton Tower (II) | | 7 | | Sat 05.01.01 | Luton Town (qSL) | 1-2 | Bristol Rovers (qSL) | | 8 | | Sat 05.01.01 | Newton Heath (II) | 3-0 | Portsmouth (SL) | | 9 | | Sat 05.01.01 | West Ham United (qSL) | 0-1 | Liverpool | | 10 |
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